Figure 3. SDF-1–induced ERM protein... | Rockefeller University Press

Figure 3.

$Figure 3. SDF-1–induced ERM protein release from the membrane-enriched fraction depends on PLC signaling. (A) Freshly isolated PBTs were pretreated with the indicated inhibitors at 37°C for 15 min, and cells were stimulated with SDF-1 at 37°C for 45 s. Subcellular fractionation by sonication and centrifugation was performed as described in Materials and methods. Total indicates postnuclear supernatant, S100 indicates soluble cytosolic fraction (S100) thereof obtained by centrifugation of postnuclear supernatant at 100,000 g, and P100 indicates pellet (P100) whose enrichment in membrane is documented by WB for MHC-I. White line indicates that intervening lanes have been spliced out. (B) Quantitative analysis of data from A by Odyssey software (version 3.0). SDF-1 stimulation induces release of both moesin and ezrin from membrane. This release can only be prevented by PLC inhibitor U73122 but not by PI3-K inhibitor. CT, control.$

SDF-1–induced ERM protein release from the membrane-enriched fraction depends on PLC signaling. (A) Freshly isolated PBTs were pretreated with the indicated inhibitors at 37°C for 15 min, and cells were stimulated with SDF-1 at 37°C for 45 s. Subcellular fractionation by sonication and centrifugation was performed as described in Materials and methods. Total indicates postnuclear supernatant, S100 indicates soluble cytosolic fraction (S100) thereof obtained by centrifugation of postnuclear supernatant at 100,000 g, and P100 indicates pellet (P100) whose enrichment in membrane is documented by WB for MHC-I. White line indicates that intervening lanes have been spliced out. (B) Quantitative analysis of data from A by Odyssey software (version 3.0). SDF-1 stimulation induces release of both moesin and ezrin from membrane. This release can only be prevented by PLC inhibitor U73122 but not by PI3-K inhibitor. CT, control.