SDF-1–induced delocalization of ERM proteins from plasma membrane depends on PLC signaling. Immunofluorescence analysis of PBT treated as in Fig. 1. After treatments, the cells were subjected to fixation and immunofluorescence staining. The third row shows an overlay of CD44 and pERM, and the fourth row shows a higher magnification of boxed regions in the third row, which highlight either colocalization of pERM with CD44 or lack thereof. The fifth row shows differential interference contrast (DIC) images displaying cell polarization. CT, control.