Figure 2. DOCK180 is required for Rac activation and invasion in uPAR-expressing tumor cell lines. (A) BE (closed bars), MDA-MB-231 (open bars), and SNB19 (shaded bars) cells were transfected with siRNAs. NT, nontargeting control; NT-OT, ON-TARGET nontargeting control. Rac activity was quantitated at 72 h (mean + SEM; n ≥ 3). *, P < 0.05; **, P < 0.01; unpaired Student's t test. (inset) Representative immunoblots from one Rac pull down in BE cells. Irrelevant lanes were removed (represented by vertical black lines). (B) BE (closed bars) and MDA-MB-231 (open bars) cells transfected with siRNAs were assayed for collagen-I invasion (mean + SEM; n ≥ 3). *, P < 0.05; **, P < 0.01; unpaired Student's t test. Immunoblots showing knockdown are in Fig. S2 A, available.
Figure 2.

DOCK180 is required for Rac activation and invasion in uPAR-expressing tumor cell lines. (A) BE (closed bars), MDA-MB-231 (open bars), and SNB19 (shaded bars) cells were transfected with siRNAs. NT, nontargeting control; NT-OT, ON-TARGET nontargeting control. Rac activity was quantitated at 72 h (mean + SEM; n ≥ 3). *, P < 0.05; **, P < 0.01; unpaired Student's t test. (inset) Representative immunoblots from one Rac pull down in BE cells. Irrelevant lanes were removed (represented by vertical black lines). (B) BE (closed bars) and MDA-MB-231 (open bars) cells transfected with siRNAs were assayed for collagen-I invasion (mean + SEM; n ≥ 3). *, P < 0.05; **, P < 0.01; unpaired Student's t test. Immunoblots showing knockdown are in Fig. S2 A.

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