Figure 1. DOCK180 is required for uPAR-driven membrane ruffling and Rac activation. (A) BE colon carcinoma cells transfected with indicated siRNAs were plated on vitronectin-coated coverslips for 12 h, fixed, and stained with Texas red–conjugated phalloidin. Bar, 50 μm. (B and C) HEK 293T cells were transfected with siRNAs targeting DOCK180 or nontargeting control (NT), and 48 h later were transfected with uPAR expression vector or empty vector control. After 24 h, Rac-GTP pull-down assays were performed. (B) Representative immunoblots to show pull-down assay, uPAR expression, and DOCK180 silencing. (C) Rac activation was quantitated and analyzed as described in Materials and methods (mean + SEM; n = 3). *, P < 0.05; **, P < 0.01; unpaired Student's t test.
Figure 1.

DOCK180 is required for uPAR-driven membrane ruffling and Rac activation. (A) BE colon carcinoma cells transfected with indicated siRNAs were plated on vitronectin-coated coverslips for 12 h, fixed, and stained with Texas red–conjugated phalloidin. Bar, 50 μm. (B and C) HEK 293T cells were transfected with siRNAs targeting DOCK180 or nontargeting control (NT), and 48 h later were transfected with uPAR expression vector or empty vector control. After 24 h, Rac-GTP pull-down assays were performed. (B) Representative immunoblots to show pull-down assay, uPAR expression, and DOCK180 silencing. (C) Rac activation was quantitated and analyzed as described in Materials and methods (mean + SEM; n = 3). *, P < 0.05; **, P < 0.01; unpaired Student's t test.

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