Figure 2.
Figure 2. The detergent-extracted EC model. (A) Schematic representation of the preparation and mechanical activation of the cell model. Confluent BAECs cultured on an elastic substrate were extracted briefly with Triton X-100. The material remaining attached was considered “the extracted cell model”. The model was subsequently incubated with buffer containing ATP and subjected to uniaxial stretch or left unstretched at 37°C. (B) Scanning electron micrographs showing the surface structure of intact cells and extracted cell models. Note that intracellular structures are exposed in extracted cells (right), whereas smooth plasma membrane is seen in intact ECs (left). Bars: (top) 10 μm; (bottom) 1 μm. (C) Immunofluorescence localization of PECAM-1 in intact and extracted BAECs. Cell border localization of PECAM-1 is preserved in the model. Bar, 10 μm.

The detergent-extracted EC model. (A) Schematic representation of the preparation and mechanical activation of the cell model. Confluent BAECs cultured on an elastic substrate were extracted briefly with Triton X-100. The material remaining attached was considered “the extracted cell model”. The model was subsequently incubated with buffer containing ATP and subjected to uniaxial stretch or left unstretched at 37°C. (B) Scanning electron micrographs showing the surface structure of intact cells and extracted cell models. Note that intracellular structures are exposed in extracted cells (right), whereas smooth plasma membrane is seen in intact ECs (left). Bars: (top) 10 μm; (bottom) 1 μm. (C) Immunofluorescence localization of PECAM-1 in intact and extracted BAECs. Cell border localization of PECAM-1 is preserved in the model. Bar, 10 μm.

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