Figure 6.
Figure 6. Two RRMs of Msi1 as a regulated modifier domain of its cytoplasmic localization. Illustration of Msi1 variants that were modifications of the constructs described in Fig. 2 A. (B) HeLa cells were transfected with constructs expressing Flag-Msi1, Flag-Msi1ABmut, 3xFlag-Msi1-D2 (1–189 and 235–362), T7-Msi1, T7-Msi1Amut, T7-Msi1Bmut, T7-Msi1CdelG (1–234), and T7-Msi1CdelI (1–189). HeLa cells treated with (44°C for 30 min; left panels) or without (right panels) heat stress were stained with anti-Flag (green), anti-T7 (green), anti-eIF4G (red), and anti-Dcp1a (red, C) antibodies, respectively. Nuclei were stained with Hoechst (blue) in the merged images. The white arrowheads and white arrow indicate PBs and SGs, respectively. Bars, 5 μm.

Two RRMs of Msi1 as a regulated modifier domain of its cytoplasmic localization. Illustration of Msi1 variants that were modifications of the constructs described in Fig. 2 A. (B) HeLa cells were transfected with constructs expressing Flag-Msi1, Flag-Msi1ABmut, 3xFlag-Msi1-D2 (1–189 and 235–362), T7-Msi1, T7-Msi1Amut, T7-Msi1Bmut, T7-Msi1CdelG (1–234), and T7-Msi1CdelI (1–189). HeLa cells treated with (44°C for 30 min; left panels) or without (right panels) heat stress were stained with anti-Flag (green), anti-T7 (green), anti-eIF4G (red), and anti-Dcp1a (red, C) antibodies, respectively. Nuclei were stained with Hoechst (blue) in the merged images. The white arrowheads and white arrow indicate PBs and SGs, respectively. Bars, 5 μm.

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