Effects of a cytochalasin B and a gelsolin fragment on the diffusion of YE(gly)2. (A) Effects of cytochalasin B (cyto) and jasplakinolide (jas) on the autocorrelation of YE(gly)2. Cells expressing YE(gly)2 were pretreated with vehicle, 1 μM jasplakinolide, or 20 μM cytochalasin B for 15 min. The obtained G(τ) is shown with 95% confidence interval. The number of measurements was 25 for vehicle, 52 for jasplakinolide, and 18 for cytochalasin B. The best fit is shown (right). (B) Alteration of filamentous YFP-actin in a single cell by cytochalasin B treatment for 15 min. (C) Effects of Ch-gelsolN on YFP-actin. Ch-gelsolN was transiently expressed for 16 h in cells stably expressing YFP-actin. Single asterisks indicate cells expressing Ch-gelsolN, double asterisks indicate a rounded cell extensively expressing Ch-gelsolN, and the arrowhead indicates filamentous actin. (D) G(τ) of YE(gly)2 in cells coexpressing YE(gly)2 with Ch-gelsolN or Ch. The number of measurements was 52 for Ch-gelsolN and 21 for Ch. The best fit is shown. Bars, 5 μm.
