Figure 7. Simultaneous observation of YE(gly)2 and ERESs. (A) YE(gly)2 (green) was coexpressed in cells stably expressing Sec13-mStrawberry (Sc13mS; red). The two fluorescent proteins were simultaneously recorded and are shown as single channels and merged images. Whole-cell images of Sc13mS are shown (left). In contrast to the reticular distribution of YE(gly)2, Sc13mS remained as an almost identical punctuate structure throughout the recording (projection). (B) Kymograph of a single pixel, which is indicated by the dashed lines in A. Here, the Sc13mS signal appeared as a line. (C) Enlarged images of the dashed boxes in A and B.
Figure 7.

Simultaneous observation of YE(gly)2 and ERESs. (A) YE(gly)2 (green) was coexpressed in cells stably expressing Sec13-mStrawberry (Sc13mS; red). The two fluorescent proteins were simultaneously recorded and are shown as single channels and merged images. Whole-cell images of Sc13mS are shown (left). In contrast to the reticular distribution of YE(gly)2, Sc13mS remained as an almost identical punctuate structure throughout the recording (projection). (B) Kymograph of a single pixel, which is indicated by the dashed lines in A. Here, the Sc13mS signal appeared as a line. (C) Enlarged images of the dashed boxes in A and B.

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