Figure 3. Diffusional motion of ER lumenal proteins is markedly affected by the attachment of two N-linked oligosaccharides. (A) The fluorescence of cells expressing each cargo was measured at 32°C. Mean G(τ) was obtained from 34 (YE; blue) or 58 (YE(gly)2; red) measurements. Means (line) and 95% confidence intervals (vertical bars) are shown. (inset) Fitting to a three-component model. Red crosses, averaged G(τ); blue dots, best fit. (B) Best-fit values of diffusion time and each subpopulation fraction. (C) Estimation of hydrodynamics diameter. Normalized G(t) was averaged from 10 cell lysates and is shown with 95% confidence intervals. YE, blue; YE(gly)2, red. The best fit in a one-component model was 0.064 μs and 0.074 μs for YE and YE(gly)2, respectively.
Figure 3.

Diffusional motion of ER lumenal proteins is markedly affected by the attachment of two N-linked oligosaccharides. (A) The fluorescence of cells expressing each cargo was measured at 32°C. Mean G(τ) was obtained from 34 (YE; blue) or 58 (YE(gly)2; red) measurements. Means (line) and 95% confidence intervals (vertical bars) are shown. (inset) Fitting to a three-component model. Red crosses, averaged G(τ); blue dots, best fit. (B) Best-fit values of diffusion time and each subpopulation fraction. (C) Estimation of hydrodynamics diameter. Normalized G(t) was averaged from 10 cell lysates and is shown with 95% confidence intervals. YE, blue; YE(gly)2, red. The best fit in a one-component model was 0.064 μs and 0.074 μs for YE and YE(gly)2, respectively.

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