Effects of cisplatin on MCF-7 cellular cytoskeleton. (A) MCF-7 cells were grown on 10-cm dishes up to 60% confluency. Then cells were treated with either cisplatin (5 μg/ml) or DMSO for 2 h. Cell morphology was examined under an inverted light microscope equipped with a CCD camera using 20x objectives. Representative areas are enlarged in the insets. (B) Cells treated as in A were fixed and stained with rhodamine-phalloidin. (C) Cells treated with vehicle (DMSO), phalloidin, cytochalasin D, or cisplatin (60 and 120 min) were analyzed for filamentous (F) and globular (G) actin by Western blotting. Results were quantitated by densitometry using NIH ImageJ. Figures are representative of at least three independent experiments.