Translocation of Smo and Ptc in and out of the primary cilium after SAG stimulation. (A) Localization of anti-Smo (green) to the primary cilium of LRB003 cells (tb, red, arrows) at 0 h of SAG treatment. (B and C) Localization of anti-Smo to the primary cilium (arrows) at 1 and 4 h, respectively. The insets in A–C show high resolution (shifted overlays) of Smo (green) in the primary cilium (red). The asterisk indicates the base of the cilium. (D) Localization of anti-Ptc (green) to the primary cilium (tb, red, arrows) at 0 h of SAG treatment. (E and F) Localization of anti-Ptc at 1 and 4 h, respectively, to primary cilium (arrows). The insets in D–F show high resolution images (shifted overlays) of Ptc (green) in the primary cilium (red). Nuclei were stained with DAPI.