A summary of Rab GEFs indicating their target Rabs. Human (Homo sapiens, hs), fruit fly (Drosophila melanogaster, dm), and nematode (C. elegans, ce) Rabs and budding yeast (Saccharomyces cerevisiae, sc) Ypts were aligned using ClustalX and plotted using NJplot (Larkin et al., 2007). The alignment is annotated to show the known Rab GEFs: TRAPP, Sec2, the Vps9 domain family, Ric1-Rgp1, Mon1-Ccz1, claret, and the DENN domain family. Images to the right indicate the typical localization of the DENN domain family in HeLa cells. The pattern of conservation is summarized in the text to the right. The accession nos. for human, mouse, zebrafish, fruit fly, and nematode DENN domains proteins are listed in Table S1. Bar, 10 µm.