Figure 5.
Figure 5. MyoD negatively regulates Pax3, Bcl-2, and Bcl-xL expression. (A) MyoD, Bcl-2, Bcl-xL, Pax3, and Pax7 gene expression were compared between wild-type myoblasts infected with a lentivirus vector expressing shRNA vector for MyoD and a control shRNA vector (Cont) or MyoD−/− myoblasts infected with a lentivirus vector expressing MyoD (Ect-MyoD) and a control empty vector by RT-PCR. (B) Bcl-2, Bcl-xL, and Pax3 protein expression were compared between wild-type myoblasts infected with a lentivirus vector expressing shRNA vector for MyoD and a control shRNA vector or MyoD−/− myoblasts infected with a lentivirus vector expressing MyoD and a control empty vector by Western blotting. (A and B) β-Actin was monitored as a loading control. (C) Relative Pax3, Bcl-2, and Bcl-xL protein expression levels normalized by β-actin expression were measured by Western blotting. *, P < 0.05; **, P < 0.01. Error bars indicate SEM.

MyoD negatively regulates Pax3, Bcl-2, and Bcl-xL expression. (A) MyoD, Bcl-2, Bcl-xL, Pax3, and Pax7 gene expression were compared between wild-type myoblasts infected with a lentivirus vector expressing shRNA vector for MyoD and a control shRNA vector (Cont) or MyoD−/− myoblasts infected with a lentivirus vector expressing MyoD (Ect-MyoD) and a control empty vector by RT-PCR. (B) Bcl-2, Bcl-xL, and Pax3 protein expression were compared between wild-type myoblasts infected with a lentivirus vector expressing shRNA vector for MyoD and a control shRNA vector or MyoD−/− myoblasts infected with a lentivirus vector expressing MyoD and a control empty vector by Western blotting. (A and B) β-Actin was monitored as a loading control. (C) Relative Pax3, Bcl-2, and Bcl-xL protein expression levels normalized by β-actin expression were measured by Western blotting. *, P < 0.05; **, P < 0.01. Error bars indicate SEM.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal