Figure 2. Expression of MyoD induces apoptosis. (A) Caspase-3 activity was compared between wild-type (wt) and MyoD−/− myoblasts under differentiation conditions from day 0 to 5 or UV exposure or treatment with thapsigargin from 0 to 24 h. (B) Under differentiation conditions from day 0 to 5, caspase-3 activity was compared between MyoD−/− myoblasts infected with a lentivirus vector expressing ectopic MyoD and a control empty vector. (C) After UV exposure or treatment with thapsigargin (Thap) for 1 d, caspase-3 activity was compared between MyoD−/− myoblasts infected with a lentivirus vector expressing ectopic MyoD and a control empty vector. (D and E) Under differentiation conditions from day 0 to 5 or after UV exposure or treatment with thapsigargin (Thap) for 1 d, cell death (D) and caspase-3 activity (E) were compared between wild-type myoblasts infected with a lentiviral vector expressing shRNA for MyoD and a control shRNA vector. *, P < 0.05; **, P < 0.01. Error bars indicate SEM.
Figure 2.

Expression of MyoD induces apoptosis. (A) Caspase-3 activity was compared between wild-type (wt) and MyoD−/− myoblasts under differentiation conditions from day 0 to 5 or UV exposure or treatment with thapsigargin from 0 to 24 h. (B) Under differentiation conditions from day 0 to 5, caspase-3 activity was compared between MyoD−/− myoblasts infected with a lentivirus vector expressing ectopic MyoD and a control empty vector. (C) After UV exposure or treatment with thapsigargin (Thap) for 1 d, caspase-3 activity was compared between MyoD−/− myoblasts infected with a lentivirus vector expressing ectopic MyoD and a control empty vector. (D and E) Under differentiation conditions from day 0 to 5 or after UV exposure or treatment with thapsigargin (Thap) for 1 d, cell death (D) and caspase-3 activity (E) were compared between wild-type myoblasts infected with a lentiviral vector expressing shRNA for MyoD and a control shRNA vector. *, P < 0.05; **, P < 0.01. Error bars indicate SEM.

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