Blmcko/neo spermatocytes show defects in γH2AX accumulation. Spermatocytes from Blmcko/neo mice display abnormal γH2AX localization in pachynema when stained with antibodies against SYCP3 (red), γH2AX (green), DAPI (blue), and CREST (pink). γH2AX should be limited to the sex body and minor regions of accumulation on the chromosome cores at this stage in wild type (Fernandez-Capetillo et al., 2003); however, the staining in these cells is excessive. (insets) Enlarged regions of staining are shown. Note the complex chromatin configuration in the first two SYCP3 insets, and the bridge of SYCP3 staining is shown by the arrow. (right) A mass of cells stained similar to wild type, surrounding one cell showing complete overaccumulation of γH2AX and complex chromosome configurations. Dashed lines show the region that is enlarged in the indicated inset.
