Figure 7. BTfnR-containing CCPs recruit more AP-2 complexes and are larger than residual CCPs. Intensity analyses based on the LCa-EGFP (A and B) or σ2-EGFP (C and D) channels in cells incubated with either A1 (A and C; blue) or A4 (B and D; red). BTfnR-containing CCPs (solid lines) were parsed out from residual CCPs (dotted lines) for five lifetime cohorts: 11–20, 21–40, 41–60, 61–80, and 81–100 s. (E) Maximum σ2-EGFP intensity plateau for each cohort (average of top five intensities ± SD) of residual (shaded blue) and bTfnR-containing (solid blue) CCPs for A1-treated cells. (F) Maximum σ2-EGFP intensity plateau of CCP cohorts in A1- (blue) and A4 (red)-containing CCPs. For LCa-EGFP, A1: NCCP = 28,382; Ncell = 5. A4: NCCP = 46,508; Ncell = 9. For σ2-EGFP, A1: 25,670; Ncell = 6. A4: 21,028; Ncell = 6. **, P < 0.01.
Figure 7.

BTfnR-containing CCPs recruit more AP-2 complexes and are larger than residual CCPs. Intensity analyses based on the LCa-EGFP (A and B) or σ2-EGFP (C and D) channels in cells incubated with either A1 (A and C; blue) or A4 (B and D; red). BTfnR-containing CCPs (solid lines) were parsed out from residual CCPs (dotted lines) for five lifetime cohorts: 11–20, 21–40, 41–60, 61–80, and 81–100 s. (E) Maximum σ2-EGFP intensity plateau for each cohort (average of top five intensities ± SD) of residual (shaded blue) and bTfnR-containing (solid blue) CCPs for A1-treated cells. (F) Maximum σ2-EGFP intensity plateau of CCP cohorts in A1- (blue) and A4 (red)-containing CCPs. For LCa-EGFP, A1: NCCP = 28,382; Ncell = 5. A4: NCCP = 46,508; Ncell = 9. For σ2-EGFP, A1: 25,670; Ncell = 6. A4: 21,028; Ncell = 6. **, P < 0.01.

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