Distinct dynamic behaviors of bTfnR-containing CCPs. (A) Segregation of CCPs into bTfnR-containing and residual CCPs. (Left panel) Shown is the accumulation of AF568-A1 in five lifetime cohorts within the ensemble of EGFP-Cla–labeled CCPs. (Right panel) Cargo-based deconvolution was used to parse ensemble CCPs into either bTfnR-containing (blue) or residual CCPs (dotted). (B and C) Fraction of CCPs found in each lifetime cohort for bTfnR-containing and residual CCPs for A1- and A4-treated cells, respectively. The fraction unaccounted for in the summations are CCPs with lifetimes >100 s. All pairwise comparison CCP fractions in all lifetime cohorts were significantly different as determined by paired t test (P < 0.05), except for the 40–60-s cohort for A1-treated cells. (D and E) Survival functions of CCPs in cells expressing bTfnR and incubated with either A1 (D, blue) or A4 (E, red). BTfnR-containing and residual CCPs are shown in solid and dotted lines, respectively. A1: N_CCP = 28,382; N_cell = 5. A4: N_CCP = 46,508; N_cell = 9.