Figure 5. Internalization of A4 is more rapid than A1. (A) Uptake assay of AF647 A4 (blue) and A1 (red) in bTfnR-expressing LCa-EGFP BSC1 cells measured by flow cytometry (n = 7, average ± SD). For each experiment, the fraction internalized was normalized to the uptake of A1 at 10 min in order to compare multiple experiments. *, P < 0.05 by paired t test. (B) Competition of AF647 A1 uptake by the presence of different unlabeled SAs as measured by flow cytometry of 10,000 cells per condition. Error bars denote SEM.
Figure 5.

Internalization of A4 is more rapid than A1. (A) Uptake assay of AF647 A4 (blue) and A1 (red) in bTfnR-expressing LCa-EGFP BSC1 cells measured by flow cytometry (n = 7, average ± SD). For each experiment, the fraction internalized was normalized to the uptake of A1 at 10 min in order to compare multiple experiments. *, P < 0.05 by paired t test. (B) Competition of AF647 A1 uptake by the presence of different unlabeled SAs as measured by flow cytometry of 10,000 cells per condition. Error bars denote SEM.

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