Figure 4. CCP density increases with bTfnR clustering. (A) Box-plot of CCP density in bTfnR-expressing cells incubated with different SAs (at least 10 regions of interest from different cells). (B) Initiation density of CCPs as determined from TIRF imaging of bTfnR-expressing cells (n = 10–16) incubated with different SAs. *, P < 0.05 for t test. (C) Accumulation of AF568-A1 and LCa-EGFP in A1-treated cells in five lifetime cohorts (10–19, 20–39, 40–59, 60–79, and 80–99 s) within the ensemble of CCPs. AF568A1 (dotted line) and LCa-EGFP (blue line) accumulated together (yellow region). (D) Accumulation of AF568-A4 and LCa-EGFP in A4-treated cells. AF658A4 (dotted line) was found to precede clathrin assembly (red line), as highlighted in the yellow region. A1: NCCP = 28,382; Ncell = 5. A4: NCCP = 46,508; Ncell = 9.
Figure 4.

CCP density increases with bTfnR clustering. (A) Box-plot of CCP density in bTfnR-expressing cells incubated with different SAs (at least 10 regions of interest from different cells). (B) Initiation density of CCPs as determined from TIRF imaging of bTfnR-expressing cells (n = 10–16) incubated with different SAs. *, P < 0.05 for t test. (C) Accumulation of AF568-A1 and LCa-EGFP in A1-treated cells in five lifetime cohorts (10–19, 20–39, 40–59, 60–79, and 80–99 s) within the ensemble of CCPs. AF568A1 (dotted line) and LCa-EGFP (blue line) accumulated together (yellow region). (D) Accumulation of AF568-A4 and LCa-EGFP in A4-treated cells. AF658A4 (dotted line) was found to precede clathrin assembly (red line), as highlighted in the yellow region. A1: NCCP = 28,382; Ncell = 5. A4: NCCP = 46,508; Ncell = 9.

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