Atg8 homologue–binding-deficient mutants of OATL1 were localized at the Golgi apparatus instead of at autophagosomes. Wild-type MEF cells transiently expressing GFP-OATL1 (top), GFP–OATL1-WA (middle), or GFP–OATL1-ED/AA (bottom) under starved conditions were fixed and stained with anti-LC3 antibody and anti-GM130 antibody. Merged images between GFP-OATL1, LC3, and GM130 are shown on the right. The insets show magnified views of the boxed areas. Bar, 10 µm.