The spatial relationship between the Golgi and the centrosome during the mammalian cell cycle. Golgi (red, stained with antibodies to GM130) and centrosome (green, stained with antibodies to centrin) staining of nonsynchronized bone cancer cells (U2-OS) shows the physical proximity between these two organelles during interphase (left) and its temporary loss during mitosis (right). Bar, 10 µm.