Tmod3 and -4 substitute for Tmod1 by capping thin filament pointed ends in adult skeletal muscle. (A) Western blots of 1-mo-old EDL muscle tissue lysates show no apparent changes in Tmod3 or -4 levels in the absence of Tmod1. Tubulin and actin were loading controls. Molecular mass is indicated in kilodaltons. (B–D) EDL muscle from Tmod1^+/+ and Tmod1^−/−Tg+ mice stained for F-actin and either Tmod1 (B), -3 (C), or -4 (D). Bars, 5 µm.