Alterations in chromatin modifications in condensin KD ES cells. (A) H3K9me3 localization in control (mock KD) and condensin KD (Smc2 KD) E14 ES cells, as shown by immunofluorescence. The arrowhead indicates a cell with large pericentric heterochromatin H3K9me3 foci. (B) Quantification of control and condensin KD E14 (WT) and p53^−/− ES cells with large foci of H3K9me3 staining. Means and standard deviations of three replicate experiments are shown. (C) 5meC in control (mock KD) and condensin KD (Smc2 KD) E14 ES cells. The arrowhead indicates a cell with large 5meC foci. (D) Quantification of 5meC staining, as in B. (E) H3S10P localization in control and condensin KD E14 ES cells. The green arrowhead indicates a cell with diffuse or weak H3S10P interphase staining. The red arrowhead indicates an interphase cell with small H3S10P foci (a pattern similar to that is transiently seen when somatic cells are serum stimulated). The blue arrowhead indicates an interphase cell with large H3S10P foci at pericentric heterochromatin. (F) Percentage of E14 (WT) and p53^−/− ES cells in each category indicated on the right. Bars, 5 µm.