Condensin KD results in interphase chromatin decondensation. (A) DNA-FISH for Mecp2 and Pgk1 in control (mock) or condensin KD (Smc2 and Smc4 KD) E14 ES cells. The merged panel (right) consists of Mecp2 (red), Pgk1 (green), and DAPI (blue). (B) Area occupied by Mecp2, Pgk1, Fn1, or OR2-1 loci in control and condensin KD ES cells, as measured by FISH. The maximum area occupied by each of the four loci was plotted against nuclear diameter for 10–20 cells per locus for control (mock) and condensin KD E14 ES cells. Bars, 5 µm.