GnT1IP-L localizes to the ER, ERGIC, and Golgi. (A–C) HeLa cells transiently expressing different GnT1IP constructs were fixed, immunolabeled for Myc-tagged GnT1IP-L (red) and GM130 or PDI (green), or HA-tagged GnT1IP-L (green) and ERGIC-53 (red), followed by confocal microscopy (A and C) or fluorescence microscopy (B). (D) HeLa cells transiently expressing HA-GnT1IP-L or GlcNAcT-I-HA were treated with CHX for 45 min followed by BFA for 30 min, fixed, immunolabeled for HA-GnT1IP-L or GlcNAcT-I-HA (green) and ERGIC-53 (red), and analyzed by fluorescence microscopy. Bars, 10 µm.