Figure 3. Conventional secretory pathway is not required for Acb1 secretion. (A) Yeast were grown in rich medium at 25°C, washed, and starved for 4 h in acetate buffer at the permissive (25°C) or restrictive temperature (37°C). SDF-2–like activity was determined in the bioassay. The data show an average of three experiments. (B) Cells from A were lysed and Western blotted with anti-Pgk1 and anti-Acb1 antibodies to monitor protein levels at the permissive and nonpermissive temperature.
Figure 3.

Conventional secretory pathway is not required for Acb1 secretion. (A) Yeast were grown in rich medium at 25°C, washed, and starved for 4 h in acetate buffer at the permissive (25°C) or restrictive temperature (37°C). SDF-2–like activity was determined in the bioassay. The data show an average of three experiments. (B) Cells from A were lysed and Western blotted with anti-Pgk1 and anti-Acb1 antibodies to monitor protein levels at the permissive and nonpermissive temperature.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal