Phosphorylation by PKC regulates EBP50’s ability to maintain microvilli. (A) Results from scoring JEG-3 cells for the presence of microvilli; error bars indicate mean ± SD. (B) Representative images from the microvillar rescue assay. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green). Bar, 10 µm.