Figure 5. Phosphorylation by Cdc2 inhibits EBP50’s role in forming microvilli in interphase but not mitotic cells. (A) Results from scoring JEG-3 cells for the presence of microvilli; error bars indicate mean ± SD. (B) Representative images from the microvillar rescue assay. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green). Bar, 10 µm. (C) JEG-3 cells transfected with siRNA targeting EBP50 (siEBP50) or luciferase (siControl) were arrested in 50 ng/ml nocodazole overnight, fixed and stained for endogenous EBP50 (red), ezrin (green), and DNA (blue). Note the loss of EBP50 from microvilli of the cell cortex in siEBP50-treated cells, whereas ezrin localization remains unchanged. Bar, 5 µm.
Figure 5.

Phosphorylation by Cdc2 inhibits EBP50’s role in forming microvilli in interphase but not mitotic cells. (A) Results from scoring JEG-3 cells for the presence of microvilli; error bars indicate mean ± SD. (B) Representative images from the microvillar rescue assay. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green). Bar, 10 µm. (C) JEG-3 cells transfected with siRNA targeting EBP50 (siEBP50) or luciferase (siControl) were arrested in 50 ng/ml nocodazole overnight, fixed and stained for endogenous EBP50 (red), ezrin (green), and DNA (blue). Note the loss of EBP50 from microvilli of the cell cortex in siEBP50-treated cells, whereas ezrin localization remains unchanged. Bar, 5 µm.

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