Figure 3. The EBP50–ezrin interaction is critical for microvilli. GFP-tagged RNAi-resistant mutants of EBP50 were expressed in EBP50 siRNA (siEBP50)–treated JEG-3 cells and their abilities to maintain microvilli were assayed. (A) Results from scoring cells for the presence of microvilli; error bars indicate mean ± SD. (B) Representative maximum projection images of the apical surface from the microvillar rescue assay. Top row was not treated with siEBP50, and shows control cells transfected to express the GFP-Nuc control plasmid. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green). Microvilli were visualized on the apical surface of cells by ezrin and phalloidin staining. Bar, 10 µm.
Figure 3.

The EBP50–ezrin interaction is critical for microvilli. GFP-tagged RNAi-resistant mutants of EBP50 were expressed in EBP50 siRNA (siEBP50)–treated JEG-3 cells and their abilities to maintain microvilli were assayed. (A) Results from scoring cells for the presence of microvilli; error bars indicate mean ± SD. (B) Representative maximum projection images of the apical surface from the microvillar rescue assay. Top row was not treated with siEBP50, and shows control cells transfected to express the GFP-Nuc control plasmid. Cells were stained for ezrin (red) and actin (blue) and transfected cells identified as those expressing the GFP-tagged construct (green). Microvilli were visualized on the apical surface of cells by ezrin and phalloidin staining. Bar, 10 µm.

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