Depletion of EBP50 by siRNA causes a defect in microvilli formation. JEG-3 cells were treated with siRNA against luciferase as a control (A–C) or against EBP50 (D–F) and examined by scanning electron microscopy (SEM; A, B, D, and E) or confocal microscopy by ezrin staining (C and F). Low and high magnification SEM images were acquired at 4,000 and 20,000x, respectively. Control cells showed numerous microvilli, whereas siEBP50 cells showed a dramatic reduction in microvilli by both SEM and ezrin staining. Confocal images were processed to be shown at the same scale as the high magnification SEM micrographs.