Requirement for paxillin and Hic5 for adhesion formation. (A) Immunoblot analysis of paxillin (Pxn; left), Hic5 (right), and tubulin (Tub; both) in lysates of mock-transfected cells or cells transfected with siRNA pools against paxillin, Hic5, or both. (B) Immunofluorescence localization of Hic5 (red), paxillin (red), and vinculin (Vcl; green) in control cells or cells transfected with siRNA pools against paxillin or Hic5. (C) Immunofluorescence localization of paxillin (red) and vinculin, talin 1 (Tln), or PY epitopes (P-Tyr; green) in control cells or cells transfected with siRNA pools against both paxillin and Hic5. (B and C) Merged images of boxed regions are magnified in third columns. Bars, 2 µm.