Interaction between hMis14 and HP1 in interphase. (A and B) Immunoprecipitation (IP) of GFP-tagged hMis14 (A) and HP1-α (B). Cultures of HeLa cells that stably expressed GFP-tagged hMis14 or HP1-α were used: AS culture is predominantly interphase, and nocodazole-arrested (Noc) culture is mitotically arrested. (C) Interphase HeLa cells that stably expressed GFP-hMis14 were stained with antibodies against HP1-α (top). Metaphase spread chromosomes of HeLa cells stably expressing GFP–HP1-α were stained with anti-hMis14 antibodies (bottom). Cross section images were deconvolved and stacked. Insets show a higher magnification view of the boxed areas. Bars, 10 µm.