Quantitative analysis of growth cone–substrate adhesiveness. The adhesiveness of growth cones expressing the indicated transgene products was measured by blasting assays. Blast durations required to dislodge growth cones are shown as dot blots. The duration value was taken as 500 ms when a growth cone had not been dislodged by a 500-ms blast. Each circle corresponds to a single growth cone. Horizontal bars indicate the median and the interquartile range. Numbers in parentheses indicate the total number of growth cones examined. ***, P < 0.001 (Dunn’s multiple comparison test).