Full-length myosin Va or Vb rescues the filopodial rotation in neurons expressing the myosin Va head domain. (A–D) The line sketches in each panel show trajectories of filopodial tips of a single growth cone that expresses MyoVaHD-Venus plus MyoVa/IRES/mRFP (A), MyoVaHD-Venus plus MyoVb/IRES/mRFP (B), MyoVaHD-Venus plus MyoVc/IRES/mRFP (C), or MyoVaHD-Venus plus mRFP (D). Each color corresponds to a single filopodium. All of the filopodial tips that appeared in the focal plane for a period of 5-min imaging were included in this study. The numbered end of each line represents the point where a filopodial tip first appeared in the focal plane, and the other end of the line is the point at which it moved out of the focal plane. The mean angular velocity (ω) and the mean velocity (v) of filopodial tips for each growth cone are shown. Positive values of the angular velocity indicate right-screw rotation.