Figure 4.
Figure 4. FYCO1 colocalizes with Rab7 and decorates the limiting membranes of autophagosomes, LEs, and lysosomes. (A) HeLa cells transfected with the indicated constructs were imaged by confocal microscopy 24 h after transfection. Insets show an enlarged field of interest. (B–D) Immunoelectron micrographs of HeLa cells transfected with GFP-FYCO1 and stained with anti-GFP polyclonal antibody (10-nm gold particle). Bars: (A) 10 µm; (A, insets) 1 µm.

FYCO1 colocalizes with Rab7 and decorates the limiting membranes of autophagosomes, LEs, and lysosomes. (A) HeLa cells transfected with the indicated constructs were imaged by confocal microscopy 24 h after transfection. Insets show an enlarged field of interest. (B–D) Immunoelectron micrographs of HeLa cells transfected with GFP-FYCO1 and stained with anti-GFP polyclonal antibody (10-nm gold particle). Bars: (A) 10 µm; (A, insets) 1 µm.

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