mtm and class II Pi3K68D interact for normal hemocyte protrusions and distribution. (A) Microtubules (green) are shown. 95% Kc₁₆₇ cells elongated 1 d after ecdysone. mtm RNAi blocked elongation (4%) and was reverted upon Pi3K68D coRNAi (56%) and less so by Vps34 coRNAi (36%). (B) F-actin in hemocytes (top) and zoom of cell edges (bottom). Pi3K68D, mtm coknockdown rescued lack of hemocyte protrusions (UAS-IRPi3K68D¹⁶²⁴⁰, UAS-IRmtm^3.5/Pxn-GAL4). Weakly modified or persistent lack of protrusions with coexpression of mtm RNAi and Vps34-KD (UAS-Vps34-KD^m8, UAS-IRmtm^3.1/+; Pxn-GAL4/+) or in double mutants (mtm^Δ77, Vps34^Δm22/mtm^z2-4747, Vps34^Δm22). (C) GFP-positive hemocytes (Pxn-GAL4) in intact larvae. mtm and Pi3K68D coRNAi reverted clumped to normal hemocyte distribution. Boxed areas are shown in higher magnification on the bottom.