Channel properties of Isoform B coexpressed with Isoform B_Δ4. (A and B) Currents from the coexpression of ANO2 Isoforms B and B_Δ4 at holding potentials of −40 and +40 mV in response to varying concentrations of Ca²⁺. (C) Comparison of current inactivation of the coexpression (seven patches) with that of Isoform B alone across all Ca²⁺ concentrations (data for Isoform B replotted from Fig. 2 G for comparison). *, P < 0.01; **, P < 0.0001; two-way ANOVA. (D and E) Hill fits of normalized current values from the coexpression of Isoforms B and B_Δ4 at holding potentials of −40 and +40 mV (seven patches). Hill fits of currents from Isoform B (from Fig. 2, E and F) are also drawn for comparison. (F) I-V relationship at saturating Ca²⁺ for the coexpression and its comparison with Isoform B.