Channel properties of Isoform A coexpressed with Isoform A_Δ4. (A and B) Currents from the coexpression of ANO2 Isoforms A and A_Δ4 at holding potentials of −40 and +40 mV in response to varying concentrations of Ca²⁺. (C) Comparison of current inactivation of the coexpression with that of Isoform A alone across all Ca²⁺ concentrations (data for A replotted from Fig. 2 G for comparison). *, P < 0.01; two-way ANOVA. (D and E) Hill fits of normalized current values from the coexpression of Isoforms A and A_Δ4 at holding potentials of −40 and +40 mV (eight and six patches, respectively). Hill fits of currents from Isoform A (from Fig. 2, E and F) are also drawn for comparison. (F) I-V relationship at saturating Ca²⁺ for the coexpression and its comparison with Isoform A.