Changes in blood and hematopoietic progenitor cells in NFAT5-deficient mice upon acute LCMV infection. (A–C) Changes in the abundance of the indicated blood (A) and bone marrow (B) cell populations and percentage of LSK and HSCs in cell cycle phases (C) in wild-type mice through 7 d after acute LCMV infection (LCMV strain Docile; 2 × 10² PFU per mouse). Bone marrow cell numbers in B and E were counted from two femurs and two tibiae per mouse. Results in A–C are from one LCMV infection experiment with four independent mice per day. (D) Changes in the cellularity of blood and bone marrow in wild-type and Nfat5^fl/fl LysM-Cre mice 3 d after acute LCMV infection. (E) Changes in the cellularity of hematopoietic bone marrow progenitors and quiescence (Ki67 staining) in LSK and HSCs in wild-type and Nfat5^fl/fl LysM-Cre mice 3 d after acute LCMV infection. Dots in the graphics in D and E represent individual mice. Results in D and E are from one to two uninfected mice of each genotype, and nine Nfat5^+/+ LysM-Cre and eight Nfat5^fl/fl LysM-Cre LCMV-infected mice. Statistical significance was determined with an unpaired t test. *, P < 0.05.