Transcriptional and post-transcriptional regulation of AP1 factors in keratinocytes. (A) Genome browser tracks at the JUN locus showing normalized ChIP-seq reads for Mi-2β in NHKs and in the HaCaT cells, and for JUN, H3K27Ac, RNApII, and H3K4me3 in CTR (black histogram) and Mi2KD (red histogram) NHKs. (B) Genome browser tracks at the Junb locus showing normalized ATAC-seq reads from ex vivo WT and Mi2Δ keratinocytes, and ChIP-seq for Mi-2β in primary mouse keratinocytes. (C and D) Expression of JUN, JUNB, JUND, and FOS mRNAs (P values as determined by DESeq2; C) and immunoblotting for p-c-JUN, c-JUN, JUNB, FOS, Mi-2β, and YY1 (loading CTR; D) in CTR and Mi2KD primary NHKs at days 3 and 5 after shRNA lentiviral transduction. The experimental procedure is described in Fig. 5 E. (E and F) Expression of JunB mRNA (P value as determined by DESeq2; E) and immunoblotting of JunB, p-c-Jun, c-Jun, and YY1 (loading control; F) in WT, Mi2Δ, and TS (6, 24, and 96 h) ex vivo mouse keratinocytes. (G) Immunohistochemistry for c-Jun in WT and Mi2Δ epidermis. Scale bar = 10 µm. Arrows point to nuclei with c-Jun expression. ChIP-seq, ATAC-seq, and RNA-seq were performed as described in Figs. 1, 3, 4, and 5. Immunoblot data shown in D and F are representative of three independent experiments. Immunostaining of c-Jun shown in G is representative of three independent experiments with three WT and three Mi2Δ mice.