Barrier disruption-induced chromatin changes and associated TFs. (A) Experimental approach for ATAC studies after barrier disruption or Mi-2β depletion in keratinocytes. Back skin was harvested before and at the indicated time points after TS or Mi-2β depletion, and sorted keratinocytes were processed for ATAC-seq. (B) dATACi or dATACl peaks in TS or Mi2Δ keratinocytes compared with WT keratinocytes are shown by histograms (6 h: red; 24 h: pink; and Mi2Δ: green). (C) Genomic annotation of dATACi peaks at 6 or 24 h after TS or in Mi2Δ keratinocytes revealed a similar genome-wide distribution. (D) Distribution of dATACi changes (log₂ of dATACi peak score [PS]) at annotated genomic locations in TS (6 or 24 h) or Mi2Δ keratinocytes. dATACi peaks were subdivided into four subsets with low to high peak scores (0–1, 1–2, 2–3, 3+), represented by color shading within the respective histograms. FC, fold change. (E) De novo motif (Homer) discovery reveals potential TF association with dATACi peaks in TS (6 or 24 h) or Mi2Δ keratinocytes. P value for motif discovery and % of peaks (targets) with motif are shown. (F) Read density (reads per bp per peak) at dATACi peaks with AP1 motif detected in TS (6 or 24 h) or Mi2Δ keratinocytes relative to WT. ATAC-seq datasets were generated from two independent experiments with pooled samples at 0 h (n = 4), 6 h (n = 4), and 24 h (n = 4) and from Mi2Δ mice (n = 4) in A–F.