Common transcription changes induced by stress signaling and Mi-2 β depletion. (A) MA (log₂ ratio of expression change versus mean average expression) plot of differentially expressed genes (|log₂ fold change| ≥1, FDR <0.05) at 6 or 24 h after TS. Mean normalized read counts (log₂ transformed) are plotted against log₂ fold change. Shared up- or down-regulated genes with Mi2Δ keratinocytes are highlighted in red and blue, respectively. (B) The number of commonly up- (red) or down-regulated (blue) genes after TS (6 or 24 h) and in Mi2Δ keratinocytes is shown. A highly significant concordance of differentially expressed genes under the two conditions was calculated by Fisher’s exact test (P < 0.0001). The percentage of grand total is shown in parentheses. (C) The overlap in differentially expressed genes after TS (|log₂ fold change| ≥1, FDR <0.05) or in Mi2Δ keratinocytes is shown by Venn diagrams. Up-regulated genes (UP) are shown at the top and down-regulated genes (DOWN) at the bottom. (D) GSEA comparing significantly up- or down-regulated genes at 6 h (top) or 24 h (bottom) after TS with the transcriptional profile of Mi2Δ keratinocytes. Enrichment profile is plotted, and calculated ES and FDR are shown. Analysis was performed with RNA-seq datasets generated from the biological replicates described in Fig. 1 and in Kashiwagi et al. (2017).