Figure S3.
Macrophage responses at sterile injuries in response to MYDGF depletion. (A and B) Representative brightfield images (A) and quantification (B) of tail fin regrowth area following thermal injury of the caudal fin of larvae microinjected at the posterior caudal vein with PBS or clodronate liposomes (Cld); three independent replicates with n = 48 PBS and 49 clodronate liposomes at 1 d postburn (dpb), 47 PBS and 47 clodronate liposomes at 2 dpb, 46 PBS and 47 clodronate liposomes at 3 dpb, and 44 PBS and 45 clodronate liposomes at 4 dpb; scale bar = 100 µm. P values were calculated by unpaired two-tailed t test; *, P < 0.05. (C) Quantification of the number of macrophages at the wound microenvironment at 4 and 24 hpw following tail transection of the caudal fin of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 21 +/+ and 30 −/− at 4 hpw, 20 +/+ and 38 −/− at 24 hpw. P values were calculated by ANOVA with Tukey’s multiple comparisons. (D) Quantification of the number of physical contact between neutrophils and macrophages at the wound microenvironment over the course of time-lapse imaging following tail transection of the caudal fin of 3 dpf WT and mydgf−/− larvae; four independent replicates with n = 7 +/+ and 11 −/− larvae; see Video 1. (E and F) Quantification of the number of macrophages (E) and the number of physical contacts between neutrophils and macrophages (F) at the burn microenvironment over the course of time-lapse imaging following thermal injury of the caudal fin of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 10 +/+ and 10 −/− larvae; see Video 2. The number of contacts displayed in D and F are adjusted values, where the raw number of contacts was multiplied by the number of neutrophils and normalized to number of macrophages at each frame. No statistical differences were detected, as calculated by unpaired two-tailed t test. In B and C, data are expressed as mean with 95% CI; each symbol represents one larva, and different colors represent the results of three independent replicates.

Macrophage responses at sterile injuries in response to MYDGF depletion. (A and B) Representative brightfield images (A) and quantification (B) of tail fin regrowth area following thermal injury of the caudal fin of larvae microinjected at the posterior caudal vein with PBS or clodronate liposomes (Cld); three independent replicates with n = 48 PBS and 49 clodronate liposomes at 1 d postburn (dpb), 47 PBS and 47 clodronate liposomes at 2 dpb, 46 PBS and 47 clodronate liposomes at 3 dpb, and 44 PBS and 45 clodronate liposomes at 4 dpb; scale bar = 100 µm. P values were calculated by unpaired two-tailed t test; *, P < 0.05. (C) Quantification of the number of macrophages at the wound microenvironment at 4 and 24 hpw following tail transection of the caudal fin of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 21 +/+ and 30 −/− at 4 hpw, 20 +/+ and 38 −/− at 24 hpw. P values were calculated by ANOVA with Tukey’s multiple comparisons. (D) Quantification of the number of physical contact between neutrophils and macrophages at the wound microenvironment over the course of time-lapse imaging following tail transection of the caudal fin of 3 dpf WT and mydgf−/− larvae; four independent replicates with n = 7 +/+ and 11 −/− larvae; see Video 1. (E and F) Quantification of the number of macrophages (E) and the number of physical contacts between neutrophils and macrophages (F) at the burn microenvironment over the course of time-lapse imaging following thermal injury of the caudal fin of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 10 +/+ and 10 −/− larvae; see Video 2. The number of contacts displayed in D and F are adjusted values, where the raw number of contacts was multiplied by the number of neutrophils and normalized to number of macrophages at each frame. No statistical differences were detected, as calculated by unpaired two-tailed t test. In B and C, data are expressed as mean with 95% CI; each symbol represents one larva, and different colors represent the results of three independent replicates.

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