MYDGF depletion impairs neutrophil reverse migration and resolution following injury. (A and B) Representative images (A) and quantification (B) of green (total) and red (photoconverted) dendra2-labeled neutrophils in the wound microenvironment at 6 hpw following tail transection of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 33 +/+ and 36 −/−; scale bar = 100 µm. (C) Quantification of the percentage of photoconverted neutrophils present at the wound at 2 hpw that are no longer present at 6 hpw in the same larva. (D and E) Representative images (D) and quantification (E) of green (total) and red (photoconverted) dendra2-labeled neutrophils in the burn at 24 hpb following thermal injury of the caudal fin of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 20 +/+ and 15 −/−; scale bar = 100 µm. (F) Quantification of the percentage of photoconverted neutrophils present in the burn microenvironment at 3 hpb that are no longer present at 24 hpb in the same larva. (G) Representative images of immunostaining for active caspase-3 (casp3) following tail transection of 3 dpf WT and mydgf−/− larvae with mCherry-labeled neutrophils at 6 hpw; scale bar = 100 µm. (H) Quantification of total and active caspase-3–expressing neutrophils in the wound at 6 hpw; three independent replicates with n = 42 +/+ and 71 −/−. (I) Proportion of neutrophils in the wound expressing active caspase-3 at 6 hpw. In B, C, E, F, H, and I, data are expressed as mean with 95% CI; each symbol represents one larva, and different colors represent the results of three independent replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P values were calculated by ANOVA with Tukey’s multiple comparisons.
Figure 4.

MYDGF depletion impairs neutrophil reverse migration and resolution following injury. (A and B) Representative images (A) and quantification (B) of green (total) and red (photoconverted) dendra2-labeled neutrophils in the wound microenvironment at 6 hpw following tail transection of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 33 +/+ and 36 −/−; scale bar = 100 µm. (C) Quantification of the percentage of photoconverted neutrophils present at the wound at 2 hpw that are no longer present at 6 hpw in the same larva. (D and E) Representative images (D) and quantification (E) of green (total) and red (photoconverted) dendra2-labeled neutrophils in the burn at 24 hpb following thermal injury of the caudal fin of 3 dpf WT and mydgf−/− larvae; three independent replicates with n = 20 +/+ and 15 −/−; scale bar = 100 µm. (F) Quantification of the percentage of photoconverted neutrophils present in the burn microenvironment at 3 hpb that are no longer present at 24 hpb in the same larva. (G) Representative images of immunostaining for active caspase-3 (casp3) following tail transection of 3 dpf WT and mydgf−/− larvae with mCherry-labeled neutrophils at 6 hpw; scale bar = 100 µm. (H) Quantification of total and active caspase-3–expressing neutrophils in the wound at 6 hpw; three independent replicates with n = 42 +/+ and 71 −/−. (I) Proportion of neutrophils in the wound expressing active caspase-3 at 6 hpw. In B, C, E, F, H, and I, data are expressed as mean with 95% CI; each symbol represents one larva, and different colors represent the results of three independent replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P values were calculated by ANOVA with Tukey’s multiple comparisons.

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