mydgf expression in sterile injuries and infection. (A) Expression of mydgf, measured by RNA sequencing (fragments per kilobase of transcript per million mapped reads [fpkm]), in three cell types is displayed 3 h following multiple wounding along the tail fin tissue. Each dot represents one independent replicate. Sample preparation and RNA-sequencing dataset was published previously (Houseright et al., 2020). (B and C) RT-qPCR measurement of mydgf expression in WT zebrafish tails 3 h following tail transection (Tt) or tail burn wound (burn; B) and P. aeruginosa (Pa; C) otic infection at 2 h after infection. Data comprise three (burn and infection) to five (Tt) independent experiments performed in technical triplicates and are normalized to mydgf expression in unwounded (Unwnd) tails and to ef1α. n = 50 tails per condition per independent replicate. *, P < 0.05. Fold changes in gene expression were compared with the normalized value of 1 using one-sample t tests. Data are displayed as mean with 95% CI.