STING has not influence on autophagosome initiation and canonical autophagy regulators. (A–C) STING WT or KO MEF cells were treated with Torin 1 for 3 h before immunostaining by anti-WIPI2 (A), FIP200 (B), or ATG16L (C) primary antibodies and secondary antibodies. Images were taken by confocal microscopy. Representative images were shown on left panels. Right panels show the quantification of puncta number for the indicated proteins. Scale bar, 10 µm. Data are represented as mean ± SD for at least 30 cells. NS, not significant. Unpaired two-tailed t test for comparison of two groups. (D) STING WT and STING KO MEFs were transfected with GFP-DFCP1 for 24 h, then treated with Torin 1 for 3 h before fluorescence of GFP-DFCP1 was imaged by confocal microscopy. Representative images were shown on left panels. Right panels show the quantification of puncta number of the indicated proteins. Data are represented as mean ± SD for at least 30 cells. NS, not significant. Unpaired two-tailed t test for comparison of two groups. (E) Canonical autophagy regulator expression level analysis in STING WT or STING KO MEF cells. STING WT and STING KO MEF cells were collected and lysed for Western blotting by the indicated antibodies. (F) Quantification of three independent repeats of E. Data are represented as mean ± SD. NS, not significant; ST, STING. Unpaired two-tailed t test for comparison of two groups. Source data are available for this figure: SourceData FS2.
Figure S2.

STING has not influence on autophagosome initiation and canonical autophagy regulators. (A–C) STING WT or KO MEF cells were treated with Torin 1 for 3 h before immunostaining by anti-WIPI2 (A), FIP200 (B), or ATG16L (C) primary antibodies and secondary antibodies. Images were taken by confocal microscopy. Representative images were shown on left panels. Right panels show the quantification of puncta number for the indicated proteins. Scale bar, 10 µm. Data are represented as mean ± SD for at least 30 cells. NS, not significant. Unpaired two-tailed t test for comparison of two groups. (D) STING WT and STING KO MEFs were transfected with GFP-DFCP1 for 24 h, then treated with Torin 1 for 3 h before fluorescence of GFP-DFCP1 was imaged by confocal microscopy. Representative images were shown on left panels. Right panels show the quantification of puncta number of the indicated proteins. Data are represented as mean ± SD for at least 30 cells. NS, not significant. Unpaired two-tailed t test for comparison of two groups. (E) Canonical autophagy regulator expression level analysis in STING WT or STING KO MEF cells. STING WT and STING KO MEF cells were collected and lysed for Western blotting by the indicated antibodies. (F) Quantification of three independent repeats of E. Data are represented as mean ± SD. NS, not significant; ST, STING. Unpaired two-tailed t test for comparison of two groups. Source data are available for this figure: SourceData FS2.

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