Elimination of syx17 abolishes STING-induced increase of autophagic flux in Drosophila fat body. (A) Micrographs of fat bodies from 92–94-h-old size-matched fed larvae with or without CQ (3 mg/ml) for 24 h from the indicated genotypes. Projections of three sections of confocal micrographs encompassing six to eight cells stained for Atg8a. Scale bar, 20 µm. (B) Quantification of Atg8a puncta in fat bodies averaged from 15 or more fed larvae from three experiments with or without CQ treatment. ****, P ≤ 0.0001; NS, not significant. (C) Micrographs of fat bodies from 92–94-h-old size-matched starved larvae with or without CQ (3 mg/ml) for 24 h from indicated genotypes. Projections of three sections of confocal micrographs encompassing six to eight or cells stained for Atg8a. Scale bar, 20 µm. (D) Quantification of Atg8a puncta in fat bodies averaged from 15 or more staved larvae from three experiments with or without CQ treatment. ****, P ≤ 0.0001; NS, not significant.