Figure S1.
KKT2 fragments localize at kinetochores from S phase to anaphase, while KKT3 central domain localizes constitutively.(A–C) Ectopically expressed TbKKT2 central domain (562–677), TbKKT2672–1030, and TbKKT2 DPB (1,024–1,260) localize at kinetochores from S phase until anaphase. (D) Ectopically expressed TbKKT3 central domain (594–728) forms kinetochore-like dots throughout the cell cycle. Inducible GFP-NLS fusion proteins were expressed with 10 ng/ml doxycycline. tdTomato-KKT1 was used as a kinetochore marker (n = 25 in each cell cycle stage). Cell lines: BAP1998, BAP2000, BAP1999, and BAP1997. Scale bars, 5 µm.

KKT2 fragments localize at kinetochores from S phase to anaphase, while KKT3 central domain localizes constitutively. (A–C) Ectopically expressed TbKKT2 central domain (562–677), TbKKT2672–1030, and TbKKT2 DPB (1,024–1,260) localize at kinetochores from S phase until anaphase. (D) Ectopically expressed TbKKT3 central domain (594–728) forms kinetochore-like dots throughout the cell cycle. Inducible GFP-NLS fusion proteins were expressed with 10 ng/ml doxycycline. tdTomato-KKT1 was used as a kinetochore marker (n = 25 in each cell cycle stage). Cell lines: BAP1998, BAP2000, BAP1999, and BAP1997. Scale bars, 5 µm.

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