pERMs negatively regulate myosin contractility. (A) Representative phase-contrast still images from Video 2 showing the effect of 10 nM calyculin A on wild-type or knockout cells. (B) Same as A, except for 30-min treatment with 25 µM blebbistatin (Video 3) or transfection to express phosphomimetic ezrin-T567E (Video 4) before calyculin A treatment. Scale bars, 100 µm.