Effects of actin-polymerizing drugs on Jeg-3 epithelial cells. (A) Jeg-3 cells treated with 1 µg/ml cytochalasin D for 30 min before fixation and staining with ZO-1 and actin. (B) Actin staining of apical and basolateral slices from maximum-intensity projection images in Fig. 5 A. Notably, latrunculin B treatment favors disassembly of basolateral actin over apical actin while maintaining a strong presence of junctional actin. Scale bars, 10 µm.